New super-resolution method for imaging in live cells

Post date: Sep 05, 2020 1:4:6 PM

We have published a new approach to super-resolution imaging in live cells. Entitled "LIVE-PAINT", the method was developed in collaboration with Lynne Regan's lab in the School of Biology. LIVE-PAINT takes advantage of peptide-protein interactions; the protein-of-interest is tagged with a short peptide sequence, which has a binding partner fused to a fluorescence protein. As the fluorescent protein and peptide transiently bind, a fluorescent burst results, and this is accurately pinpointed with nanometre accuracy. Repeated binding events allow a super-resolution image to be built from this.

Well done to Curran, Zoe, Louise, Owen and Simon!