Publications
2023
Colom-Cadena, M., Davies, C., Sirisi, S., Lee, J.-E., Simzer, E., Tzioras, M., Querol-Vilaseca, M., Sánchez-Aced, É., Chang, Y. Y., Holt, K., McGeachan, R., Rose, J., Tulloch, J., Wilkins, L., Smith, C., Andrian, T., Belbin, O., Pujals, S., Horrocks, M. H., Lleó, A., Spires-Jones, T.
Neuron, 111, 1–14, 2023. http://dx.doi.org/10.1016/j.neuron.2023.04.020
Jeacock, K., Chappard, A., Gallagher, K. J., Mackay, C. L., Kilgour, D. P. A., Horrocks, M. H., Kunath, T., Clarke, D. J.
Journal of the American Society for Mass Spectrometry, Just published, 2023. http://dx.doi.org/10.1021/jasms.2c00339
Single-Molecule Two-Color Coincidence Detection of Unlabeled alpha-Synuclein Aggregates
Chappard, A., Leighton, C., Saleeb, R.S., Jeacock, K., Ball, S.R., Morris, K., Kantelberg, O., Lee, J., Zacco, E., Pastore, A., Sunde, M., Clarke, D.J., Downey, P., Kunath, T., Horrocks, M.H.
Angewandte Chemie International Edition, e202216771, 2023. http://dx.doi.org/10.1002/anie.202216771
2022
Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach
Bhaskar, H., Kleinjan, D.J., Oi, C., Gidden, Z., Rosser, S., Horrocks, M.H.*, Regan, L.*, *Joint corresponding authors
Protein Science, e4558, 2022. http://dx.doi.org/10.1002/pro.4558
Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging
de Moliner, F., Konieczna, Z., Mendive-Tapia, L., Saleeb, R.S., Morris, K., Gonzalez-Vera, J.A., Kaizuka, T., Grant, S.G.N., Horrocks, M.H.*, Vendrell, M.*, *Joint corresponding authors
Angewandte Chemie International Edition, e202216231, 2022. http://dx.doi.org/10.1002/anie.202216231
Virdi, G. S., Choi, M. L., Evans, J. R., Yao, Z., Athauda, D., Strohbuecker, S., Wernick, A. I., Alrashidi, H., Melandri, D., Perez-Lloret, J., Angelova, P. R., Sylantyev, S., Eaton, S., Heales, S., Kunath, T., Horrocks, M. H., Abramov, A. Y., Patani, R., Gandhi, S.
npj Parkinson's Disease, 8, 1, 44562, 2022. http://dx.doi.org/10.1101/2022.10.28.514238
Pattle, S.B., O'Shaughnessy, J., Kantelberg, O., Rifai, O.M., Pate, J., Nellany, K., Hays, N., Arends, M.J., Horrocks, M.H., Waldron, F.M., Gregory, J.M.
The Journal of Pathology: Clinical Research, 9, 44-55, 2022. http://dx.doi.org/10.1002/cjp2.297
Structural Conversion of alpha-Synuclein at the Mitochondria Induces Neuronal Toxicity
Choi, M. L., Chappard, A., Singh, B. P., Maclachlan, C., Rodrigues, M., Fedotova, E., Berezhnov, A. V., De, S., Peddie, C., Athauda, D., Virdi, G. S., Zhang, W., Evans, J. R., Wernick, A., Zanjani, Z. S., Angelova, P. R., Esteras, N., Vinikurov, A., Morris, K., Jeacock, K., Tosatto, L., Little, D., Gissen, P., Clarke, D. J., Kunath, T., Collinson, L., Klenerman, D., Abramov, A. Y.*, Horrocks, M. H.*, Gandhi, S.*, *Joint corresponding authors
Nature Neuroscience, 25, 9, 1134-1148, 2022. http://dx.doi.org/10.1038/s41593-022-01140-3
Broadhead, M. J., Doucet, K., Kantelberg, O. G., Zhu, F., Grant, S. G. N., Horrocks, M. H., Miles, G. B.
bioRxiv , Pre-print, 2022. http://dx.doi.org/10.1101/2022.08.29.505610
Probing TDP-43 Condensation Using an in Silico Designed Aptamer.
Zacco, E., Kantelberg, O., Milanetti, E., Armaos, A., Panei, F. P., Gregory, J., Jeacock, K., Clarke, D. J., Chandran, S., Ruocco, G., Gustincich, S., Horrocks, M. H.*, Pastore, A.*, Tartaglia, G. G.*, *Joint corresponding authors
Nature Communications, 13, 1, 41275, 2022. http://dx.doi.org/10.1038/s41467-022-30944-x
Self-Assembling Protein Surfaces for In Situ Capture of Cell-Free-Synthesized Proteins.
Thornton, E. L., Paterson, S. M., Gidden, Z., Horrocks, M. H., Laohakunakorn, N., Regan, L.
Frontiers in Bioengineering and Biotechnology, 1193, 2022. http://dx.doi.org/10.3389/fbioe.2022.915035
2021
Lipid-Induced Polymorphic Amyloid Fibrils Formation by alpha-Synuclein.
Singh, B. P., Morris, R. J., Horrocks, M. H., Kunath, T., MacPhee, C. E.
bioRxiv, Pre-print, 2021. http://dx.doi.org/10.1101/2021.07.20.453062
2020
PAINT using proteins: A new brush for super-resolution artists
Oi, C., Mochrie, S.J., Horrocks, M.H., Regan, L.
Protein Science, 29, 11, 2142-2149, 2020. http://dx.doi.org/10.1002/pro.3953
Oi, C., Gidden, Z., Holyoake, L., Kantelberg, O., Mochrie, S., Horrocks, M.H.*, Regan, L.*, *Joint corresponding authors
Nature Protocols, Protocol, 2020. http://dx.doi.org/10.21203/rs.3.pex-1043/v1
Oi, C., Gidden, Z., Holyoake, L., Kantelberg, O., Mochrie, S., Horrocks, M.H.*, Regan, L.*, *Joint corresponding authors
Communications Biology, 3, 1, 45200, 2020. http://dx.doi.org/10.1038/s42003-020-01188-6
Alpha synuclein aggregation drives ferroptosis: an interplay of iron, calcium and lipid peroxidation
Angelova, P.R., Choi, M-L., Berezhnov, A.V., Horrocks, M.H., Hughes, C.D., De, S., Rodrigues, M., Yapom, R., Little, D., Dolt, K.S., Kunath, T., Devine, M.J., Gissen, P., Shchepinov, M.S., Sylantyev, S., Pavlov, E.V., Klenerman, D., Abramov, A.Y., Gandhi, S.
Cell Death and Differentiation, 27, 10, 2781-2796, 2020. http://dx.doi.org/10.1038/s41418-020-0542-z
2019
ASYN-CONA, a novel bead-based assay for detecting early stage alpha-synuclein aggregation.
Pérez-Pi, I., Evans, D.A., Horrocks, M.H., Pham, N.A., Dolt, K.S., Koszela, J., Kunath, T., Auer, M.
Analytical Chemistry, 91, 9, 5582-5590, 2019. http://dx.doi.org/10.1021/acs.analchem.8b03842
Benson, S., Fernandez, A., Barth, N., de Moliner, F., Horrocks, M.H., Herrington, S., Abad, J.L., Delgado, A., Kelly, L., Chang, Z., Feng, Y., Nishiura, M., Hori, Y., Kikuchi, K., Vendrell, M.
Angewandte Chemie International Edition, 131, 21, 6985-6989, 2019. http://dx.doi.org/10.1002/anie.201900465
2018
Mapping Surface Hydrophobicity of alpha-Synuclein Oligomers at the Nanoscale
Lee, J., Sang J.C., Rodrigues M., Carr, A.R., Horrocks, M.H., De, S., Bongiovanni, M.N., Flagmeier, P., Dobson, C.M., Wales D.J., Lee, S.F., Klenerman, D.
Nano Letters, 18, 12, 7494-7501, 2018. http://dx.doi.org/10.1021/acs.nanolett.8b02916
Nanoscopic characterization of individual endogenous protein aggregates in human neuronal cells
Whiten, D.R., Zuo, Y., Calo, L., Choi, M., De, S., Flagmeier, P., Wirthensohn, D.C., Kundel, F., Ranasinghe, R.T., Sanchez, S.E., Athauda, D., Lee, S.F., Dobson, C.M., Gandhi, S., Spillantini, M., Klenerman, D., Horrocks, M.H.
ChemBioChem, 19, 19, 2001, 2018. http://dx.doi.org/10.1002/cbic.201800209
Extrinsic Amyloid-Binding Dyes for the Detection of Individual Protein Aggregates in Solution
Taylor, C.G., Meisl, G., Horrocks, M.H., Zetterberg, H., Knowles, T.P.J., Klenerman, D.
Analytical Chemistry, 90, 17, 10385-10393, 2018. http://dx.doi.org/10.1021/acs.analchem.8b02226
Whiten, D.R., Dezerae, D., Horrocks, M.H., Taylor, C.G., De, S., Flagmeier, P., Tosatto, L., Kumita, J.R., Ecroyd, H., Dobson, C.M., Klenerman, D.
Cell Reports, 23, 12, 3492-3500, 2018. http://dx.doi.org/10.1016/j.celrep.2018.05.074
Ludtmann, M. H. R.*, Angelova, P. R.*, Horrocks, M. H.*, Choi, M. L., Rodrigues, M., Baev, A. Y., Berezhnov, A. V., Yao, Z., Little, D., Banushi, B., Al-Menhali, A.S., Ranasinghe, R.T., Whiten, D.R., Yapom, R., Dolt, K.S., Devine, M.J., Gissen, P., Kunath, T., Jaganjac, M., Pavlov, E.V., Klenerman, D., Abramov, A.Y., S. Gandhi, S., *Joint first authors
Nature Communications, 9, 1, 42370, 2018. http://dx.doi.org/10.1038/s41467-018-04422-2
A sticky situation: aberrant protein-protein interactions in Parkinson's disease
Brown, J.W.P., Horrocks, M.H.
Seminars in Cell & Developmental Biology, 99, 65-77, 2018. http://dx.doi.org/10.1016/j.semcdb.2018.05.006
Shedding light on aberrant interactions: A review of modern tools for studying protein aggregates
Kundel, F., Tosatto, L.T., Whiten, D.R., Wirthensohn, D.C., Horrocks, M.H.*, Klenerman, D.*, *Joint corresponding authors.
The FEBS Journal, 285, 19, 3604-3630, 2018. http://dx.doi.org/10.1111/febs.14409
Detecting RNA base methylations in single cells by in situ hybridization
Ranasinghe, R.T., Challand, M.R., Ganzinger, K.A., Lewis, B.W., Softley, C., Schmied, W.H., Horrocks, M.H., Shivji, N., Chin, J.W, Spencer, J., Klenerman, D.
Nature Communications, 9, 1, 45200, 2018. http://dx.doi.org/10.1038/s41467-017-02714-7
Cox, D., Whiten, D.R., Brown, J.W.P., Horrocks, M.H., San Gil, R., Dobson, C.M., Klenerman, D., van Oijen, A.M., Ecroyd, H.
Journal of Biological Chemistry, 293, 12, 4486-4497, 2018. http://dx.doi.org/10.1074/jbc.M117.813865
Hsp70 Inhibits the Nucleation and Elongation of Tau and Sequesters Tau Aggregates with High Affinity
Kundel, F., De, S., Flagmeier, P., Horrocks, M.H., Kjaergaard, M., Shammas, S.L., Jackson, S.E., Dobson, C.M., Klenerman, D.
ACS Chemical Biology, 13, 3, 636-646, 2018. http://dx.doi.org/10.1021/acschembio.7b01039
2017
Iljina, M., Hong, L, Horrocks, M.H., Ludtmann, M.H., Choi, M.L., Hughes, C.D. Ruggeri, F.S. Guilliams, T., Buell, A.K., Lee, J.E., Gandhi, S., Lee, S.F, Bryant, C.E., Vendruscolo, M., Knowles, T.P.J., Dobson, C.M., De Genst, E., Klenerman, D.
BMC Biology, 15, 1, 41640, 2017. http://dx.doi.org/10.1186/s12915-017-0390-6
Janeczek, AA, Scarpa, E., Horrocks M.H, Tare, R.S., Rowland, C.A., Jenner, D., Newman, T.A., Oreffo, R.O.C., Lee, S.F., Evans, N.D.
Nanomedicine, 12, 8, 845-863, 2017. http://dx.doi.org/10.2217/nnm-2016-0386
Co-aggregation of κ-Casein and β-Lactoglobulin Produces Morphologically Distinct Amyloid Fibrils.
Raynes, J.K., Crepin, P., Horrocks, M.H., Carver, J.A., Day, L.
Small, 13, 14, 1603591, 2017. http://dx.doi.org/10.1002/smll.201603591
2016
Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping.
Bongiovanni, M.*, Godet, J.*,Horrocks, M.H.*, Tosatto, L., Carr, A.R, Wirthensohn, D.C., Ranasinghe, R.T., Lee, J., Ponjavic, A., Fritz, J.V., Dobson, C.M., Klenerman, D., Lee, S.F., *Joint first authors
Nature Communications, 7, 1, 45170, 2016. http://dx.doi.org/10.1038/ncomms13544
Jonsson P., Southcombe J., Mafalda A., Santos A.M., Fernandes R.A., McColl J., Chang V.C., Godkin A., Dunne P.D., Horrocks M.H., Palayret M., Fugger L., Xu X., Davis S.J., Klenerman D.
Proceedings of the National Academy of Sciences, 113, 20, 5682-5687, 2016. http://dx.doi.org/10.1073/pnas.1513918113
PSD95 nanoclusters are postsynaptic building blocks in hippocampus circuits.
Broadhead M.J., Horrocks M.H., Zhu F., Muresan L., Benavides-Piccione R., DeFelipe J., Fricker D., Kopanitsa M.V., Duncan R.R., Klenerman D., Komiyama N.H., Lee S.F., Grant S.G.N.
Scientific Reports, 6, 1, 41640, 2016. http://dx.doi.org/10.1038/srep24626
A kinetic model of the aggregation of alpha-synuclein provides insights into prion-like spreading.
Iljina,M., Garciaa, G.A., Horrocks, M.H., Tosatto, L., Choi, M.L., Ganzinger, K.A., Abramov, A.Y., Gandhi, S., Wood, N.W., Cremades, N.,Dobson, C.M. , Knowles, T.P.J., Klenerman, D.
Proceedings of the National Academy of Sciences, 113, 9, E1206-E1215, 2016. http://dx.doi.org/10.1073/pnas.1524128113
Single-molecule imaging of individual amyloid protein aggregates in human biofluids.
Horrocks M. H., Lee S.F., Gandhi S., Magdalinou N.K., Chen S.W., Devine M.J., Toasatto, L., Kjaergaard M., Beckwith J.S., Zetterberg H., Iljina M., Cremades N., Dobson C.M., Wood N.W., Klenerman D.
ACS Chemical Neuroscience, 7, 3, 399-406, 2016. http://dx.doi.org/10.1021/acschemneuro.5b00324
Calcium is a key factor in alpha-synuclein induced neurotoxicity.
Angelova, P., Ludtmann, M., Horrocks, M.H., Negoda, A., Cremades, N., Klenerman, D., Dobson, C.M., Wood, N., Pavlov, E., Gandhi, S., Abramov, A.
Journal of Cell Science, 129, 9 , 1792-1801, 2016. http://dx.doi.org/10.1242/jcs.180737
2015
Deas, E., Cremades, N., Angelova, P.R., Ludtmann, M., Yao, Z., Chen, S., Horrocks, M.H., Banushi, B., Little, D., Devine, M., Gissen P., Klenerman, D., Dobson, C.M., Wood, N., Gandhi, S., Abramov. A.Y.
Antioxidants and redox signaling, 24, 7, 376-391, 2015. http://dx.doi.org/10.1089/ars.2015.6343
Horrocks, M.H., Tosatto, L., Dear, A.J., Knowles, T.P.J., Dalla Serra, M., Dobson, C.M., Klenerman, D.
Scientific Reports, 5, 1, 45261, 2015. http://dx.doi.org/10.1038/srep16696
Horrocks, M.H., Tosatto, L., Dear, A.J., Garcia, G.A., Iljina, M., Cremades, N., Dalla Serra, M., Knowles, T.P.J., Dobson, C.M., Klenerman, D.
Analytical Chemistry, 87, 17, 8818-8826, 2015. http://dx.doi.org/10.1021/acs.analchem.5b01811
Reeve, A.K., Ludtmann, M.H.R, Angelova, P.R., Simcox, E.M., Horrocks, M.H., Klenerman, D., Gandhi, S., Turnbull, D.M., Abramov, A.Y.
Cell Disease and Death, 6,7, e1820-e1820, 2015. http://dx.doi.org/10.1038/cddis.2015.166
Lipid peroxidation is essential for alpha-synuclein-induced cell death.
Angelova, P.R., Horrocks, M.H., Klenerman D, Gandhi S, Abramov, A.Y., Shchepinov, M.S
Journal of Neurochemistry, 133, 4, 582-589, 2015. http://dx.doi.org/10.1111/jnc.13024
A mechanistic model of tau amyloid aggregation based on direct observation of oligomers.
Shammas, S. L., Garcia, G. A., Kumar S., A., Kjaergaard M., Horrocks, M.H., Shivji N., Mandelkow, E., Knowles T.P.J., Mandelkow E., Klenerman, D..
Nature Communications, 6,1, 45200, 2015. http://dx.doi.org/10.1038/ncomms8025
2014
Murphy, R.M., Danezis, D., Horrocks, M.H., Jackson, S.E., Klenerman, D.
Analytical Chemistry, 86, 17, 8603-8612, 2014. http://dx.doi.org/10.1021/ac501188r
The changing point-spread function: single-molecule-based super-resolution imaging.
Horrocks, M.H., Palayret, M., Klenerman, D., Lee, S.F.
Histochemistry and Cell Biology, 141, 6, 577-585, 2014. http://dx.doi.org/10.1007/s00418-014-1186-1
2013
Single-molecule measurements of transient biomolecular complexes through microfluidic dilution
Horrocks, M.H., Rajah, L., Jonsson, P., Kjaergaard, M., Vendruscolo, M., Knowles, T.P.J., Klenerman, D.
Analytical Chemistry, 85, 14, 6855-6859, 2013. http://dx.doi.org/10.1021/ac4010875
2012
Ubiquitin chain conformation regulates recognition and activity of interacting proteins.
Ye, Y., Blaser, G., Horrocks, M.H., Ruedas-Rama, M.J., Ibrahim, S., Zhukov, A.A., Orte, A., Klenerman, D., Jackson, S.E., Komander, D.
Nature, 492, 7428, 266-270, 2012. http://dx.doi.org/10.1038/nature11722
Single molecule fluorescence under conditions of fast flow.
Horrocks, M.H., Li, H., Shim, J.-U., Ranasinghe, R.T., Clarke, R.W., Huck, W.T.S., Abell, C., Klenerman, D.
Analytical Chemistry, 84, 1, 179-185, 2012. http://dx.doi.org/10.1021/ac202313d