Publications
2024
Daly, S., Bulovaite, E., Handa, A., Morris, K., Muresan, L., Adams, C., Kaizuka, T., Kitching, A., Spark, A., Chant, G., O'Holleran, K., Grant, S. G. N., Horrocks, M. H., Lee, S. F.
bioRxiv, Pre-print, 2024. http://dx.doi.org/10.1101/2024.10.14.617231
Salis Torres, A., Lee, J.-E., Caporali, A., Semple, R. K., Horrocks, M. H., MacRae, V. E.
International Journal of Molecular Sciences, 25 (20), 10973., 2024. http://dx.doi.org/10.3390/ijms252010973
57. A long fluorescence lifetime probe for labeling of Gram-negative bacteria
Baibek, A., Konieczna, Z., Ucuncu, M., Alghamdi, Z.S., Sharma, R., Horrocks, M.H., Bradley M.
chemRxiv, Pre-print, 2024. http://dx.doi.org/10.26434/chemrxiv-2024-f0rjq
56. Super-resolution imaging of proteins inside live mammalian cells with mLIVE-PAINT
Bhaskar, H., Gidden, Z., Virdi, G., Kleinjan, D., Rosser, S.J., Gandhi, S., Regan, L., Horrocks, M.H.
bioRxiv, Pre-print, 2024. http://dx.doi.org/10.1101/2024.07.22.604574
55. Sequential replacement of PSD95 subunits in postsynaptic supercomplexes is slowest in the cortex
Morris, K., Bulovaite, E., Kaizuka, T., Schnorrenberg, S., Adams, C., Komiyama, N., Mendive-Tapia, L., Grant, S. G. N., Horrocks, M. H.
eLife, Reviewed Preprint, 2024. http://dx.doi.org/10.7554/eLife.99303.1
Zepernick, A., Metodieva, V., Pelegrina-Hidalgo, N., Lippert, A.H., Horrocks, M.H., Varela, J.A.
Nanoscale, 16, 9576-9582, 2024. http://dx.doi.org/10.1039/d4nr00330f
Spence, H., Waldron, F. M., Saleeb, R. S., Brown, A.-L., Rifai, O. M., Gilodi, M., Read, F., Roberts, K., Milne, G., Wilkinson, D., O'Shaughnessy, J., Pastore, A., Fratta, P., Shneider, N., Tartaglia, G. G., Zacco, E., Horrocks, M. H., Gregory, J. M.
Acta Neuropathologica, 147, 50, 2024. http://dx.doi.org/10.1007/s00401-024-02705-1
Athauda, D., Evans, J. R., Mahoney-Sanchez, L., Virdi, G. S., Lopez-Garcia, P., Wernick, A., Wagen, A., D'Sa, K., Lachica, J., Strohbuecker, S., Vecchi, G., Leighton, C., Saleeb, R. S., O'Shaughnessy, J., Toomey, C. E., Vijiaratnam, N., Girges, C., Li, Y., Mustapic, M., Ismail, K., Davies, M., Kapogiannis, D., Choi, M. L., Ryten, M., Horrocks, M. H., Greig, N., Foltynie, T., Gandhi, S.
bioRxiv, Pre-print, 2024. http://dx.doi.org/10.1101/2024.02.28.582460
2023
Saleeb, R.S., Leighton, C., Lee, J., O'Shaughnessy, J., Jeacock, K., Chappard, A., Cumberland, R., Zhao, T., Ball, S.R., Sunde, M., Clarke, D.J., Piche, K., McPhail, J.A., Louwrier, A., Angers, R., Gandhi, S., Downey, P., Kunath, T., Horrocks, M.H.
Science Advances, 9, eadi7359, 2023. http://dx.doi.org/10.1126/sciadv.adi7359
50. Imaging Proteins Sensitive to Direct Fusions Using Transient Peptide-Peptide Interactions.
Gidden, Z., Oi, C., Johnston, E.J., Konieczna, Z., Bhaskar, H., Mendive-Tapia, L., de Moliner, F., Rosser, S.J., Mochrie, S. G. J., Vendrell, M., Horrocks, M. H., Regan, L.
ACS Nano Letters, 23, 22, 10633-10641, 2023. http://dx.doi.org/10.1021/acs.nanolett.3c03780
Broadhead, M. J., Ayvazian-Hancock, A., Doucet, K., Kantelberg, O. G., Zhu, F., Motherwell, L., Grant, S. G. N., Horrocks, M. H., Miles, G. B.
Frontiers in Molecular Neuroscience, 16, 2023. http://dx.doi.org/10.3389/fnmol.2023.1027898
48. Prediction of mechanistic subtypes of Parkinson's using patient derived stem cell models
D, Karishma, Evans, J.R., Virdi, G.S., Vecchi, G., Adam, A., Bertolli, O., Fleming, J., Chang, H., Leighton, C., Horrocks, M.H., Athauda, D., Choi, M.L., Gandhi, S.
Nature Machine Intelligence, 5, 933-946, 2023. http://dx.doi.org/10.1038/s42256-023-00702-9
47. Lipid-Induced Polymorphic Amyloid Fibrils Formation by alpha-Synuclein.
Singh, B. P., Morris, R. J., Kunath, T., MacPhee, C. E., Horrocks, M. H.
Protein Science, 32:e4736, 2023. http://dx.doi.org/10.1002/pro.4736
Colom-Cadena, M., Davies, C., Sirisi, S., Lee, J.-E., Simzer, E., Tzioras, M., Querol-Vilaseca, M., Sanchez-Aced, E., Chang, Y. Y., Holt, K., McGeachan, R., Rose, J., Tulloch, J., Wilkins, L., Smith, C., Andrian, T., Belbin, O., Pujals, S., Horrocks, M. H., Llee, A., Spires-Jones, T.
Neuron, 111, 1-14, 2023. http://dx.doi.org/10.1016/j.neuron.2023.04.020
Jeacock, K., Chappard, A., Gallagher, K. J., Mackay, C. L., Kilgour, D. P. A., Horrocks, M. H., Kunath, T., Clarke, D. J.
Journal of the American Society for Mass Spectrometry, 34, 5, 847-856, 2023. http://dx.doi.org/10.1021/jasms.2c00339
44. Single-Molecule Two-Color Coincidence Detection of Unlabeled alpha-Synuclein Aggregates
Chappard, A., Leighton, C., Saleeb, R.S., Jeacock, K., Ball, S.R., Morris, K., Kantelberg, O., Lee, J., Zacco, E., Pastore, A., Sunde, M., Clarke, D.J., Downey, P., Kunath, T., Horrocks, M.H.
Angewandte Chemie International Edition, e202216771, 2023. http://dx.doi.org/10.1002/anie.202216771
2022
43. Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach
Bhaskar, H., Kleinjan, D.J., Oi, C., Gidden, Z., Rosser, S., Horrocks, M.H.*, Regan, L.*, *Joint corresponding authors
Protein Science, e4558, 2022. http://dx.doi.org/10.1002/pro.4558
42. Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging
de Moliner, F., Konieczna, Z., Mendive-Tapia, L., Saleeb, R.S., Morris, K., Gonzalez-Vera, J.A., Kaizuka, T., Grant, S.G.N., Horrocks, M.H.*, Vendrell, M.*, *Joint corresponding authors
Angewandte Chemie International Edition, e202216231, 2022. http://dx.doi.org/10.1002/anie.202216231
Virdi, G. S., Choi, M. L., Evans, J. R., Yao, Z., Athauda, D., Strohbuecker, S., Nirujogi, R. S., Wernick, A. I., Pelegrina-Hidalgo, N., Leighton, C., Saleeb, R. S., Kopach, O., Alrashidi, H., Melandri, D., Perez-Lloret, J., Angelova, P. R., Sylantyev, S., Eaton, S., Heales, S., Rusakov, D. A., Alessi, D. R., Kunath, T., Horrocks, M. H., Abramov, A. Y., Patani, R., Gandhi, S.
npj Parkinson's Disease, 8, 1, 44562, 2022. http://dx.doi.org/10.1038/s41531-022-00423-7
Pattle, S.B., O'Shaughnessy, J., Kantelberg, O., Rifai, O.M., Pate, J., Nellany, K., Hays, N., Arends, M.J., Horrocks, M.H., Waldron, F.M., Gregory, J.M.
The Journal of Pathology: Clinical Research, 9, 44-55, 2022. http://dx.doi.org/10.1002/cjp2.297
39. Structural Conversion of alpha-Synuclein at the Mitochondria Induces Neuronal Toxicity
Choi, M. L., Chappard, A., Singh, B. P., Maclachlan, C., Rodrigues, M., Fedotova, E., Berezhnov, A. V., De, S., Peddie, C., Athauda, D., Virdi, G. S., Zhang, W., Evans, J. R., Wernick, A., Zanjani, Z. S., Angelova, P. R., Esteras, N., Vinikurov, A., Morris, K., Jeacock, K., Tosatto, L., Little, D., Gissen, P., Clarke, D. J., Kunath, T., Collinson, L., Klenerman, D., Abramov, A. Y.*, Horrocks, M. H.*, Gandhi, S.*, *Joint corresponding authors
Nature Neuroscience, 25, 9, 1134-1148, 2022. http://dx.doi.org/10.1038/s41593-022-01140-3
38. Probing TDP-43 Condensation Using an in Silico Designed Aptamer.
Zacco, E., Kantelberg, O., Milanetti, E., Armaos, A., Panei, F. P., Gregory, J., Jeacock, K., Clarke, D. J., Chandran, S., Ruocco, G., Gustincich, S., Horrocks, M. H.*, Pastore, A.*, Tartaglia, G. G.*, *Joint corresponding authors
Nature Communications, 13, 1, 41275, 2022. http://dx.doi.org/10.1038/s41467-022-30944-x
37. Self-Assembling Protein Surfaces for In Situ Capture of Cell-Free-Synthesized Proteins.
Thornton, E. L., Paterson, S. M., Gidden, Z., Horrocks, M. H., Laohakunakorn, N., Regan, L.
Frontiers in Bioengineering and Biotechnology, 1193, 2022. http://dx.doi.org/10.3389/fbioe.2022.915035
2020
36. PAINT using proteins: A new brush for super-resolution artists
Oi, C., Mochrie, S.J., Horrocks, M.H., Regan, L.
Protein Science, 29, 11, 2142-2149, 2020. http://dx.doi.org/10.1002/pro.3953
Oi, C., Gidden, Z., Holyoake, L., Kantelberg, O., Mochrie, S., Horrocks, M.H.*, Regan, L.*, *Joint corresponding authors
Nature Protocols, Protocol, 2020. http://dx.doi.org/10.21203/rs.3.pex-1043/v1
Oi, C., Gidden, Z., Holyoake, L., Kantelberg, O., Mochrie, S., Horrocks, M.H.*, Regan, L.*, *Joint corresponding authors
Communications Biology, 3, 1, 45200, 2020. http://dx.doi.org/10.1038/s42003-020-01188-6
33. Alpha synuclein aggregation drives ferroptosis: an interplay of iron, calcium and lipid peroxidation
Angelova, P.R., Choi, M-L., Berezhnov, A.V., Horrocks, M.H., Hughes, C.D., De, S., Rodrigues, M., Yapom, R., Little, D., Dolt, K.S., Kunath, T., Devine, M.J., Gissen, P., Shchepinov, M.S., Sylantyev, S., Pavlov, E.V., Klenerman, D., Abramov, A.Y., Gandhi, S.
Cell Death and Differentiation, 27, 10, 2781-2796, 2020. http://dx.doi.org/10.1038/s41418-020-0542-z
2019
32. ASYN-CONA, a novel bead-based assay for detecting early stage alpha-synuclein aggregation.
Pérez-Pi, I., Evans, D.A., Horrocks, M.H., Pham, N.A., Dolt, K.S., Koszela, J., Kunath, T., Auer, M.
Analytical Chemistry, 91, 9, 5582-5590, 2019. http://dx.doi.org/10.1021/acs.analchem.8b03842
Benson, S., Fernandez, A., Barth, N., de Moliner, F., Horrocks, M.H., Herrington, S., Abad, J.L., Delgado, A., Kelly, L., Chang, Z., Feng, Y., Nishiura, M., Hori, Y., Kikuchi, K., Vendrell, M.
Angewandte Chemie International Edition, 131, 21, 6985-6989, 2019. http://dx.doi.org/10.1002/anie.201900465
2018
30. Mapping Surface Hydrophobicity of alpha-Synuclein Oligomers at the Nanoscale
Lee, J., Sang J.C., Rodrigues M., Carr, A.R., Horrocks, M.H., De, S., Bongiovanni, M.N., Flagmeier, P., Dobson, C.M., Wales D.J., Lee, S.F., Klenerman, D.
Nano Letters, 18, 12, 7494-7501, 2018. http://dx.doi.org/10.1021/acs.nanolett.8b02916
29. Nanoscopic characterization of individual endogenous protein aggregates in human neuronal cells
Whiten, D.R., Zuo, Y., Calo, L., Choi, M., De, S., Flagmeier, P., Wirthensohn, D.C., Kundel, F., Ranasinghe, R.T., Sanchez, S.E., Athauda, D., Lee, S.F., Dobson, C.M., Gandhi, S., Spillantini, M., Klenerman, D., Horrocks, M.H.
ChemBioChem, 19, 19, 2001, 2018. http://dx.doi.org/10.1002/cbic.201800209
28. Extrinsic Amyloid-Binding Dyes for the Detection of Individual Protein Aggregates in Solution
Taylor, C.G., Meisl, G., Horrocks, M.H., Zetterberg, H., Knowles, T.P.J., Klenerman, D.
Analytical Chemistry, 90, 17, 10385-10393, 2018. http://dx.doi.org/10.1021/acs.analchem.8b02226
Whiten, D.R., Dezerae, D., Horrocks, M.H., Taylor, C.G., De, S., Flagmeier, P., Tosatto, L., Kumita, J.R., Ecroyd, H., Dobson, C.M., Klenerman, D.
Cell Reports, 23, 12, 3492-3500, 2018. http://dx.doi.org/10.1016/j.celrep.2018.05.074
Ludtmann, M. H. R.*, Angelova, P. R.*, Horrocks, M. H.*, Choi, M. L., Rodrigues, M., Baev, A. Y., Berezhnov, A. V., Yao, Z., Little, D., Banushi, B., Al-Menhali, A.S., Ranasinghe, R.T., Whiten, D.R., Yapom, R., Dolt, K.S., Devine, M.J., Gissen, P., Kunath, T., Jaganjac, M., Pavlov, E.V., Klenerman, D., Abramov, A.Y., S. Gandhi, S., *Joint first authors
Nature Communications, 9, 1, 42370, 2018. http://dx.doi.org/10.1038/s41467-018-04422-2
25. A sticky situation: aberrant protein-protein interactions in Parkinson's disease
Brown, J.W.P., Horrocks, M.H.
Seminars in Cell & Developmental Biology, 99, 65-77, 2018. http://dx.doi.org/10.1016/j.semcdb.2018.05.006
24. Shedding light on aberrant interactions: A review of modern tools for studying protein aggregates
Kundel, F., Tosatto, L.T., Whiten, D.R., Wirthensohn, D.C., Horrocks, M.H.*, Klenerman, D.*, *Joint corresponding authors.
The FEBS Journal, 285, 19, 3604-3630, 2018. http://dx.doi.org/10.1111/febs.14409
23. Detecting RNA base methylations in single cells by in situ hybridization
Ranasinghe, R.T., Challand, M.R., Ganzinger, K.A., Lewis, B.W., Softley, C., Schmied, W.H., Horrocks, M.H., Shivji, N., Chin, J.W, Spencer, J., Klenerman, D.
Nature Communications, 9, 1, 45200, 2018. http://dx.doi.org/10.1038/s41467-017-02714-7
Cox, D., Whiten, D.R., Brown, J.W.P., Horrocks, M.H., San Gil, R., Dobson, C.M., Klenerman, D., van Oijen, A.M., Ecroyd, H.
Journal of Biological Chemistry, 293, 12, 4486-4497, 2018. http://dx.doi.org/10.1074/jbc.M117.813865
21. Hsp70 Inhibits the Nucleation and Elongation of Tau and Sequesters Tau Aggregates with High Affinity
Kundel, F., De, S., Flagmeier, P., Horrocks, M.H., Kjaergaard, M., Shammas, S.L., Jackson, S.E., Dobson, C.M., Klenerman, D.
ACS Chemical Biology, 13, 3, 636-646, 2018. http://dx.doi.org/10.1021/acschembio.7b01039
2017
Iljina, M., Hong, L, Horrocks, M.H., Ludtmann, M.H., Choi, M.L., Hughes, C.D. Ruggeri, F.S. Guilliams, T., Buell, A.K., Lee, J.E., Gandhi, S., Lee, S.F, Bryant, C.E., Vendruscolo, M., Knowles, T.P.J., Dobson, C.M., De Genst, E., Klenerman, D.
BMC Biology, 15, 1, 41640, 2017. http://dx.doi.org/10.1186/s12915-017-0390-6
Janeczek, AA, Scarpa, E., Horrocks M.H, Tare, R.S., Rowland, C.A., Jenner, D., Newman, T.A., Oreffo, R.O.C., Lee, S.F., Evans, N.D.
Nanomedicine, 12, 8, 845-863, 2017. http://dx.doi.org/10.2217/nnm-2016-0386
18. Co-aggregation of κ-Casein and β-Lactoglobulin Produces Morphologically Distinct Amyloid Fibrils.
Raynes, J.K., Crepin, P., Horrocks, M.H., Carver, J.A., Day, L.
Small, 13, 14, 1603591, 2017. http://dx.doi.org/10.1002/smll.201603591
2016
17. Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping.
Bongiovanni, M.*, Godet, J.*,Horrocks, M.H.*, Tosatto, L., Carr, A.R, Wirthensohn, D.C., Ranasinghe, R.T., Lee, J., Ponjavic, A., Fritz, J.V., Dobson, C.M., Klenerman, D., Lee, S.F., *Joint first authors
Nature Communications, 7, 1, 45170, 2016. http://dx.doi.org/10.1038/ncomms13544
Jonsson P., Southcombe J., Mafalda A., Santos A.M., Fernandes R.A., McColl J., Chang V.C., Godkin A., Dunne P.D., Horrocks M.H., Palayret M., Fugger L., Xu X., Davis S.J., Klenerman D.
Proceedings of the National Academy of Sciences, 113, 20, 5682-5687, 2016. http://dx.doi.org/10.1073/pnas.1513918113
15. PSD95 nanoclusters are postsynaptic building blocks in hippocampus circuits.
Broadhead M.J., Horrocks M.H., Zhu F., Muresan L., Benavides-Piccione R., DeFelipe J., Fricker D., Kopanitsa M.V., Duncan R.R., Klenerman D., Komiyama N.H., Lee S.F., Grant S.G.N.
Scientific Reports, 6, 1, 41640, 2016. http://dx.doi.org/10.1038/srep24626
14. A kinetic model of the aggregation of alpha-synuclein provides insights into prion-like spreading.
Iljina,M., Garciaa, G.A., Horrocks, M.H., Tosatto, L., Choi, M.L., Ganzinger, K.A., Abramov, A.Y., Gandhi, S., Wood, N.W., Cremades, N.,Dobson, C.M. , Knowles, T.P.J., Klenerman, D.
Proceedings of the National Academy of Sciences, 113, 9, E1206-E1215, 2016. http://dx.doi.org/10.1073/pnas.1524128113
13. Single-molecule imaging of individual amyloid protein aggregates in human biofluids.
Horrocks M. H., Lee S.F., Gandhi S., Magdalinou N.K., Chen S.W., Devine M.J., Toasatto, L., Kjaergaard M., Beckwith J.S., Zetterberg H., Iljina M., Cremades N., Dobson C.M., Wood N.W., Klenerman D.
ACS Chemical Neuroscience, 7, 3, 399-406, 2016. http://dx.doi.org/10.1021/acschemneuro.5b00324
12. Calcium is a key factor in alpha-synuclein induced neurotoxicity.
Angelova, P., Ludtmann, M., Horrocks, M.H., Negoda, A., Cremades, N., Klenerman, D., Dobson, C.M., Wood, N., Pavlov, E., Gandhi, S., Abramov, A.
Journal of Cell Science, 129, 9 , 1792-1801, 2016. http://dx.doi.org/10.1242/jcs.180737
2015
Deas, E., Cremades, N., Angelova, P.R., Ludtmann, M., Yao, Z., Chen, S., Horrocks, M.H., Banushi, B., Little, D., Devine, M., Gissen P., Klenerman, D., Dobson, C.M., Wood, N., Gandhi, S., Abramov. A.Y.
Antioxidants and redox signaling, 24, 7, 376-391, 2015. http://dx.doi.org/10.1089/ars.2015.6343
Horrocks, M.H., Tosatto, L., Dear, A.J., Knowles, T.P.J., Dalla Serra, M., Dobson, C.M., Klenerman, D.
Scientific Reports, 5, 1, 45261, 2015. http://dx.doi.org/10.1038/srep16696
Horrocks, M.H., Tosatto, L., Dear, A.J., Garcia, G.A., Iljina, M., Cremades, N., Dalla Serra, M., Knowles, T.P.J., Dobson, C.M., Klenerman, D.
Analytical Chemistry, 87, 17, 8818-8826, 2015. http://dx.doi.org/10.1021/acs.analchem.5b01811
Reeve, A.K., Ludtmann, M.H.R, Angelova, P.R., Simcox, E.M., Horrocks, M.H., Klenerman, D., Gandhi, S., Turnbull, D.M., Abramov, A.Y.
Cell Disease and Death, 6,7, e1820-e1820, 2015. http://dx.doi.org/10.1038/cddis.2015.166
7. Lipid peroxidation is essential for alpha-synuclein-induced cell death.
Angelova, P.R., Horrocks, M.H., Klenerman D, Gandhi S, Abramov, A.Y., Shchepinov, M.S
Journal of Neurochemistry, 133, 4, 582-589, 2015. http://dx.doi.org/10.1111/jnc.13024
6. A mechanistic model of tau amyloid aggregation based on direct observation of oligomers.
Shammas, S. L., Garcia, G. A., Kumar S., A., Kjaergaard M., Horrocks, M.H., Shivji N., Mandelkow, E., Knowles T.P.J., Mandelkow E., Klenerman, D.
Nature Communications, 6,1, 45200, 2015. http://dx.doi.org/10.1038/ncomms8025
2014
Murphy, R.M., Danezis, D., Horrocks, M.H., Jackson, S.E., Klenerman, D.
Analytical Chemistry, 86, 17, 8603-8612, 2014. http://dx.doi.org/10.1021/ac501188r
4. The changing point-spread function: single-molecule-based super-resolution imaging.
Horrocks, M.H., Palayret, M., Klenerman, D., Lee, S.F.
Histochemistry and Cell Biology, 141, 6, 577-585, 2014. http://dx.doi.org/10.1007/s00418-014-1186-1
2013
3. Single-molecule measurements of transient biomolecular complexes through microfluidic dilution
Horrocks, M.H., Rajah, L., Jonsson, P., Kjaergaard, M., Vendruscolo, M., Knowles, T.P.J., Klenerman, D.
Analytical Chemistry, 85, 14, 6855-6859, 2013. http://dx.doi.org/10.1021/ac4010875
2012
2. Ubiquitin chain conformation regulates recognition and activity of interacting proteins.
Ye, Y., Blaser, G., Horrocks, M.H., Ruedas-Rama, M.J., Ibrahim, S., Zhukov, A.A., Orte, A., Klenerman, D., Jackson, S.E., Komander, D.
Nature, 492, 7428, 266-270, 2012. http://dx.doi.org/10.1038/nature11722
1. Single molecule fluorescence under conditions of fast flow.
Horrocks, M.H., Li, H., Shim, J.-U., Ranasinghe, R.T., Clarke, R.W., Huck, W.T.S., Abell, C., Klenerman, D.
Analytical Chemistry, 84, 1, 179-185, 2012. http://dx.doi.org/10.1021/ac202313d
Patents
(WO2016001644) Diagnosis and Treatment of Neurodegenerative Disorders.
(WO2023217742A1) TDP-43-Binding Single-Stranded Aptamers and Uses Thereof.